CCR2 (C-C Chemokine Receptor Type 2, C-C CKR-2, CC-CKR-2, CCR-2, CD192, MCP-1-R, Monocyte Chemoattractant Protein 1 Receptor)

Size

£819.00

SKU:: 366932
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to human CCR2 at the N-terminal extracellular domain, fused to GST-Tag.
Clone:: 8C3.1
Format:: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Extra Details:: C-C chemokine receptor type 2 (UniProt P41597; also known as C-C CKR-2, CC-CKR-2, CCR-2, CCR2, CD192, MCP-1-R, Monocyte chemoattractant protein 1 receptor) is encoded by the CCR2 (also known as CMKBR2) gene (Gene ID 729230) in human. CC chemokine receptors (CCRs) are 7-transmembrane G protein-coupled receptors (GPCRs) that bind CC chemokines (CCLs) and mediate their biological activities. CCRs and CCLs play a central role in physiological and pathological recruitment of immune cells. CCR2 can interact with CCL2, CCL8 and CCL16, it is found on the surface of monocytes, activated memory T cells, B cells, and basophils in humans, and also in peritoneal macrophages in mice. CCR2 plays an important role in the recruitment of monocytes/macrophages and T cells. CCR2-knockout mice show a partial defect in macrophage function, decreased Th1 immune response, reduced delayed-type hypersensitivity reaction, and are more susceptible to pathogenic bacteria infections. Human CCR2 is a 7-transmembrane (aa43-70, 81-100, 115-136, 154-178, 207-226, 244-268, 286-309) GPCR with an N-terminal extracellular domain (aa1-42) and a C-terminoal cytoplasmic domain (aa310-374). Applications: Suitable for use in Western Blot and Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 2ul detects surface CCR2 expression among the gated lymphocytes population from one million human PMBCs. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice