366931
Caspr2 (Contactin-associated Protein-like 2, Cell Recognition Molecule Caspr2) [KO Validated] (Not for Export EU)
Size
£754.00
- SKU:
- 366931
- Purity:
- Serum.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein fragment corresponding to human Caspr2 extracellular domain, human IgG1 Fc-tagged.
- Format:
- Supplied as a liquid, 0.05% sodium azide.
- Extra Details:
- Contactin-associated protein-like 2 (UniProt Q9UHC6; also known as Cell recognition molecule Caspr2) is encoded by the CNTNAP2 (also known as AUTS15, CASPR2, CDFE, KIAA0868) gene (Gene ID 26047) in human. Caspr2 is a neurexin superfamily member that functions as a neuronal cell adhesion protein. Originally identified in myelinating nerves, Caspr2 serves to cluster voltage-gated potassium (Kv) channels at the juxtaparanode, a membrane domain adjacent to the node of Ranvier, where it forms a barrier holding nodal components in place. Caspr2 is found in axons, dendrites, dendritic spines and the soma of mature pyramidal neurons in the central nervous system (CNS). Caspr2 and its partner Contactin 2 are found in the synaptic plasma membranes fraction of the forebrain. In the absence of Caspr2, new synaptic spines are unable to stabilize, resulting in decreased spine density and altered spine morphology as well as downregulated AMPA receptor subunit on the spines in mice. CNTNAP2 gene mutations are linked to autism spectrum disorder (ASD) in human. Likewise, Cntnap2-knockout mice exhibit core autism-like deficits including abnormal social behavior and communication, and behavior inflexibility. Caspr2 is produced with a signal peptide (aa1-27), the removal of which yields the mature transmembrane proein with a large extracellular region (aa28-1262), followed by a transmembrane domain (aa1263-1283) and a cytoplasmic tail (human aa1284-1331 and mouse aa1284-1332). The extracellular region contains an N-terminal Discoidin domain (a.k.a. F5/8 type C domain or C2-like domain; human aa35-181), four Laminin G-like domains (human aa216-368, 401-552, 799-963, 1055-1214), two EGF-like domains (human aa554-591 and 963-1002), and a Fibrinogen C-terminal domain (human aa592-798). Applications: Suitable for use in Western Blot, Immunofluorescence and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Immunocytochemistry: 1:1000, detects Caspr2 immunoreactivity at the juxtaparanodal region around the nodes of Ranvier by indirect immunofluorescent staining of 0.5% Triton X-100-permeabilized teased fibers prepared from 4% paraformaldehyde-fixed, desheathed wild-type, but not Caspr2-knockout, mouse sciatic nerves Western Blot: 1:5000 detects Caspr2 in 10ug of mouse brain tissue lysates Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice