BCL2L12 (Bcl-2-like Protein 12)

Size

£800.00

SKU:: 366927
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein fragment corresponding to human BCL2L12 at the internal domain, fused to GST-Tag.
Clone:: 11B5.1
Format:: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Extra Details:: Bcl-2-like protein 12 (UniProt Q9HB09; also known as Bcl-2-related proline-rich protein, Bcl2-L-12) is encoded by the BCL2L12 (also known as BPR) gene (Gene ID 83596) in human. Bcl-2 family proteins paly either a pro- or anti-apoptotic role in cell death signaling. BCL-2 family members are related by regions of sequence and structural homology. The pro-survival members contain four BCL-2 homology regions (BH1 BH4), whereas the BH3-only members contain only the BH3 amphipathic helix that mediates their interaction with the groove of multi-domain BCL-2 family members. Bcl2-L-12 is an anti-apoptotic protein that shares a lower amino acid similarity with the other Bcl2 family members. It contains a BH2 (aa311-322) domain and a BH3-like domain (aa192-220) that mediate its interaction with Bcl2 and Bcl-xL. Exogenous expression of Bcl2-L-12 with Leu213 or Leu217 mutation in U87MG cell line is reported to result in reactivation of PARP cleavage, caspase-3 and cytochrome c release compared to cells expressing wild-type Bcl2-L-12. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (paraffin): 1:50 detects BCL2L12 in human pancreas, skeletal muscle, and tonsil tissue sections. Western Blot: 1:2000 detected BCL2L12 in 10ug of PC3 human prostate cancer cell lysate. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice