ARHGAP36, CT (Rho GTPase-activating Protein 36)

Size

£800.00

SKU:: 366924
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein fragment corresponding to human ARHGAP36 at C-terminal, fused to GST-Tag.
Clone:: 12D10.1
Format:: Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
Extra Details:: Rho GTPase-activating protein 36 (UniProt Q6ZRI8) is encoded by the ARHGAP36 gene (Gene ID 158763) in human. ARHGAP36 is a Rho GAP family member that acts as a potent hedgehog (Hh) pathway positive regulator. ARHGAP36 is reported to functionally associate with Hh pathway negative regulator Sufu (suppressor of fused) and ARHGAP36 overexpression results in reduced Gli3R levels, Gli2 ciliary accumulation, and Gli1 expression, in a Smo-independent manner. ARHGAP36 is produced with a signal peptide sequence (aa1-40), which is removed posttranslationally to yield the mature protein with a Rho-GAP domain (aa226-426). Unlike other Rho GAP domain-containing proteins, ARHGAP36 lacks the arginine finger motif that stimulates GTPase hydrolysis and GAP domain mutants can retain Gli-activating activity. ARHGAP36 isoforms are up-regulated in a subset of medulloblastomas, where they mediate noncanonical Gli activation by inhibiting Sufu. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (paraffin): 1:50 detects ARHGAP36 in human prostate cancer, adrenal gland and pancreas tissue sections. Western Blot: 1:500 detected 50ng of GST-tagged recombinant human ARHGAP36 C-terminal fragment. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice