366917
Actin (alpha Skeletal Muscle, Alpha-actin-1, ACTA, ACTA1)
Size
£800.00
- SKU:
- 366917
- Purity:
- Purified by affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Linear peptide corresponding to the N-terminal end sequence of mature skeletal muscle alpha-actin (ACTA1), KLH conjugated.
- Format:
- Supplied as a liquid in 0.1M Tris-Glycine, pH 7.4, 150mM sodium chloride, 0.05% sodium azide.
- Extra Details:
- Actins are globular multi-functional proteins that serve as the basic building blocks of cytoskeletal microfilaments and are among the most conserved eukaryotic proteins. Six actin types exist, skeletal muscle alpha-actin (ACTA1), smooth muscle alpha-actin (ACTA2), cytoplasmic beta-actin (ACTB), cardiac muscle alpha-actin (ACTC1), cytoplasmic gamma-actin (ACTG1), and smooth muscle gamma-actin (ACTG2, a.k.a. ACTA3). Although actins show >90% overall sequence homology, isotypes do show spatial, temporal, and tissue-specific expresson patterns and only 50-60% homology is found in their 18 N-terminal residues. Cytoplasmic - and -actins are thought to be present in all cells, while the other four actin isoforms are typically found in specific adult muscle tissue types. Actins exist in a variety of structural states, depending on the specific ionic conditions or the interaction with ligand proteins. The oligomeric and polymeric forms that actin molecules assume are dependent on the distinct conformations they adopt. In additon to serving as the basic building blocks of cytoskeletal microfilaments, actins are involved in multiple nuclear functions and adopt specific conformations that are important for their association with nuclear actin-binding proteins. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (paraffin): 1:1000 detects actin in human cardiac muscle, skeletal muscle, and small intestine tissue sections. Immunocytochemistry: 1:250 detects both cytoplasmic and nuclear actin immunoreactivity in A431 and NIH/3T3 cells. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice