365585
vWA3A (Von Willebrand Factor A Domain Containing Protein 3A)
Size
£482.00
- SKU:
- 365585
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to aa981-1176 from human vWA3A, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
- Extra Details:
- vWA3A,Contains 2 VWFA domains, Human WARP shares 79% aa identity with mouse Warp. RT-PCR of mRNA from various mouse tissues and cell lines detected Warp expression only in chondrocytes. Northern blot analysis confirmed expression of Warp in chondrocytes isolated from newborn mouse rib cartilage. Western blot analysis of transfected human embryonic kidney cells detected a 48kD protein in both cell layer and media fractions. The majority of the protein was detected in the medium, indicating that WARP is efficiently secreted. Under nonreducing conditions, a 102kD form was detected, suggesting that WARP forms a disulfide-bonded homodimer. N-glycosidase digestion led to a mobility shift from 48 to 45kD, indicating that WARP has 1 or more N-linked Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 0.01-5ug/ml Immunohistochemistry: 5-30ug/ml Immunofluorescence (IC): 5-20ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice