365584
vWA1 (Von Willebrand Factor A Domain Containing Protein 1, VWFA1, WARP)
Size
£581.00
- SKU:
- 365584
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to aa135-366 of mouse vWA1. expressed in E. coli.
- Format:
- Supplied as a liquid in 0.01M PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
- Extra Details:
- The deduced 418aa human protein contains a signal sequence, followed by a VA domain, 2 fibronectin type III (F3) repeats, and a short pro/arg-rich C terminus. The VA domain has a putative metal ion-dependent adhesion site (MIDAS) motif and 2 potential O-linked glycosylation sites, and each F3 domain has a putative N-glycosylation site. Human WARP shares 79% aa identity with mouse Warp. RT-PCR of mRNA from various mouse tissues and cell lines detected Warp expression only in chondrocytes. Northern blot analysis confirmed expression of Warp in chondrocytes isolated from newborn mouse rib cartilage. Western blot analysis of transfected human embryonic kidney cells detected a 48kD protein in both cell layer and media fractions. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.01-5ug/ml Immunohistochemistry: 5-20ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice