365545
VASN (Vasorin, SLITL2, Slit-Like 2)
Size
£537.00
- SKU:
- 365545
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to aa299-559 of mouse VASN, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- The deduced 673aa protein contains a putative hydrophobic signal sequence, 10 tandem arrays of a leucine-rich repeat, an epidermal growth factor-like domain, a fibronectin type III-like domain, and a short intracellular region. In situ hybridization detected mouse vasorin in the tunica media of the proximal ascending aorta, descending thoracic aorta, abdominal aorta, and coronary arteries. In mouse kidney, vasorin was expressed in interstitial cells. expression of vasorin in aortas increased gradually in parallel with the differentiation of vascular smooth muscle cells beginning at embryonic day 10.5. Vasorin expressed by transfected Chinese hamster ovary cells showed an apparent molecular mass of 110kD, which shifted following N-glycosidase treatment. Vasorin was expressed on the cell surface and was secreted into the culture medium Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.5-2ug/ml Immunohistochemistry (Paraffin): 5-20ug/ml Immunocytochemistry: 5-20ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice