UPb1 (Ureidopropionase Beta, UP-B1, BUP1, Beta-alanine synthase, N-carbamoyl-beta-alanine amidohydrolase)

Size

£626.00

SKU:: 365524
Purity:: Purified by Protein A and peptide affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Trp121-Glu384 of human UPb1, expressed in E. coli.
Format:: Supplied as a liquid in 0.01M PBS, pH7.4, 0.05% Proclin-300, 50% glycerol.
Extra Details:: The BUP1 open reading frame of 1,152 bp corresponds to a protein of 384aa with a calculated molecular mass of 43kD, surrounded by a 5-prime untranslated region of 61 nucleotides and a 3-prime untranslated region of 277 nucleotides. The protein shows 84% identity with the translation product of the rat beta-ureidopropionase cDNA. Expression of the human cDNA in Escherichia coli and eukaryotic COS-7 cells revealed a very high beta-ureidopropionase enzymatic activity, thus confirming the identity of the cDNA. Human EST libraries from brain, liver, kidney, and heart contained partial beta-ureidopropionase cDNAs, suggesting expression in these tissues, as seen in the rat. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice