365484
TXN2 (Thioredoxin 2, Mitochondrial, MT-TRX, TRX2, MTRX)
Size
£505.00
- SKU:
- 365484
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Thr60-Gly166 of bovine TXN2, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- Thioredoxin, mitochondrial is a mitochondrial member of the thioredoxin family, a group of small multifunctional redox-active proteins. The encoded protein may play important roles in the regulation of the mitochondrial membrane potential and in protection against oxidant-induced apoptosis. Spyrou et al. (1997) cloned rat Trx2. The deduced protein contains a mitochondrial targeting sequence and a thioredoxin active site. Northern blot analysis detected highest expression in rat heart, muscle, kidney, and adrenal gland. Western blot analysis detected Trx2 in the mitochondrial fraction of fractionated rat liver. Northern blot analysis detected TRX2 transcripts of about 1.3 and 1.0 kb in all tissues examined, with highest expression in heart, skeletal muscle, kidney, and liver. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. AA Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- T TFNIQDGPDF QDRVVNSETP VVVDFHAQWC GPCKILGPRL EKVVAKQHGK VVMAKVDIDD HTDLALEYEV SAVPTVLAMK NGDVVDKFVG IKDEDQLEAF LKKLIG Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice