365478
TUBd (Tubulin Delta, TUBD1)
Size
£626.00
- SKU:
- 365478
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Thr190-Leu453 of human TUBd, expressed in E. coli.
- Format:
- Supplied as a liquid in 0.01M PBS, pH7.4, 0.05% Proclin-300, 50% glycerol.
- Extra Details:
- The deduced 453aa protein has a calculated molecular mass of 51kD. TUBD1 contains the conserved GTP-binding domain shared with other tubulins, but it lacks the highly acidic C terminus characteristic of alpha-tubulin and beta-tubulin. Relative to other tubulins, TUBD1 also has a 5aa insertion between helices 6 and 7 and a 21-residue insertion at the end of helix 9. TUBD1 shares 40% aa identity with Chlamydomonas delta-tubulin and 85% identity with mouse delta-tubulin. Within dividing osteogenic sarcoma cells, TUBD1 partially colocalized with both centrin and gamma-tubulin, but it was most prominent between the centrioles within a centrosome. Its localization was independent of microtubules. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice