TRPM7 (Transient Receptor Potential Cation Channel Subfamily M, Member 7, CHAK, CHAK1, LTRPC7, TRP-PLIK, Channel-kinase 1, Long transient receptor potential channel 7)

Size

£577.00

SKU:: 365448
Purity:: Purified by Protein A and peptide affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Ile19-Gly179 of rat TRPM7, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: TRP-PLIK is a protein that is both an ion channel and a kinase. As a channel, it conducts calcium and monovalent cations to depolarize cells and increase intracellular calcium. As a kinase, it is capable of phosphorylating itself and other substrates. The kinase activity is necessary for channel function, as shown by its dependence on intracellular ATP and by the kinase mutants.The interaction of PLIK with PLCB1 was confirmed by coimmunoprecipitation of expressed proteins in CHO-K1 cells. Northern blot analysis revealed an approximately 8-kb transcript in brain and skeletal muscle, with stronger signals in kidney, heart, liver, and spleen, consistent with the 7,105-bp transcript size for TRP-PLIK. A hemagglutinin-tagged TRP-PLIK protein was found to be membrane bound. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice