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TRERF1 (Transcriptional Regulating Factor 1, BCAR2, TREP132, RAPA, Breast Cancer Anti-Estrogen Resistance 2, Transcriptional-regulating protein 132, Zinc finger protein rapa, Zinc finger transcription factor TReP-132)

Size

£626.00

SKU:: 365438
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Pro779-Arg936 of human TRERF1, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: Transcriptional-regulating factor 1 is a zinc-finger transcriptional regulating protein which interacts with CBP/p300 to regulate the human gene CYP11A1.The deduced 1,200aa protein contains a glutamine-rich region, 2 proline-rich regions, an acidic region, and 3 C2H2-type zinc finger motifs. An alternatively spliced transcript with a longer 3-prime UTR encodes the same protein. Northern blot analysis detected transcripts of 4.4 and 7.5 kb abundantly expressed in thymus, testis, adrenal cortex, and a human choriocarcinoma cell line. Weak expression was detected in all other tissues examined except pancreas and small intestine. In vitro transcription and translation resulted in a protein with an apparent molecular mass of about 132kD, in agreement with the predicted size. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice