SPRED2 (Sprouty Related, EVH1 Domain Containing Protein 2)

Size

£544.00

SKU:: 365161
Application:: WB
Purity:: Purified by Protein A and peptide affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Met1-Ala418 of human SPRED2, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: SPRED2 is a member of the Sprouty/SPRED family of proteins that regulate growth factor-induced activation of the MAP kinase cascade. The deduced 411aa protein contains an N-terminal Enabled (ENA)/VASP homology-1 (EVH1) domain, a central KIT-binding domain (KBD), and a C-terminal SPRY domain. Endogenous Spred2 localized to the membrane in rat PC12 cells, and mutation analysis determined that the C-terminal SPRY domain was required for plasma membrane localization.Inhibition appeared to occur through the formation of a Spred-Ras complex that inhibited activation of MAP kinase by suppressing phosphorylation and activation of Raf. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.