365157
SPON2 (Spondin 2, DIL1, Mindin, M-spondin, Extracellular Matrix Protein, Differentially expressed in cancerous and non-cancerous lung cells 1)
Size
£537.00
- SKU:
- 365157
- Application:
- WB
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Val119-Val330 of mouse SPON2, expressed in E. coli.
- Format:
- Supplied as a liquid in 0.01M PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
- Extra Details:
- The full-length SPON2 cDNA encodes a 331aa protein with a domain organization similar to those of zebrafish mindin-1/mindin-2 and F-spondin (SPON1): a hydrophobic signal sequence in the N terminus, an FS1 domain, an FS2 domain, and a thrombospondin type I repeat. The mouse mindin protein, which is 85% identical to the human protein, is a conserved member of a family of secreted extracellular matrix proteins. RNA blot analysis detected expression in a variety of tissues, with abundant expression in lung and lymphoid tissues. Immunoblot blot analysis showed expression of a 42kD protein under reducing conditions and expression of dimers and oligomers in a concentration-dependent manner under nonreducing conditions. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. AA Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- VH AVFSAPAIPS GTGQTSTELE VHPRHSLVSF VVRIVPSPDW FVGIDSLDLC EGGRWKEQVV LDLYPHDAGT DSGFTFSSPN FATIPQDTVT EITASSPSHP ANSFYYPRLK SLPPIAKVTF VRLQQSPRAF APPSLDLASR GNEIVDSLSV PETPLDCEVS LWSSWGLCGG PCGKLGAKSR TRYVRVQPAN NGTPCPELEE EAECAPDNCV Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice