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SNAPAP (SNAP Associated Protein, SNAPIN, SNAP-25-Binding Protein, Biogenesis of lysosome-related organelles complex 1 subunit 7, Synaptosomal-associated protein 25-binding protein)

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£626.00

SKU:: 365108
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Ala2-Lys136 of human SNAPAP, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: SNAPAP is a component of the SNARE complex of proteins that is required for synaptic vesicle docking and fusion. SNAPAP is also a component of the ubiquitously expressed BLOC1 multisubunit protein complex. The deduced 136aa protein has a calculated molecular mass of 15kD. It contains an N-terminal hydrophobic segment characteristic of a transmembrane domain and a coiled-coil C-terminal region. SNAPAP shares 98% sequence identity with the mouse homolog. Western blot analysis of various rat tissues showed SNAPAP almost exclusively in brain and synaptosome preparations. Further analysis indicated SNAPAP in all 12 rat brain areas examined; fractionation of rat cerebral synaptosomes indicated that SNAPAP partitions with the synaptic vesicle fraction and not with cytosolic or plasma membrane fractions. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice