365104
SNAI1 (Snail Homolog 1, SLUGH2, SNA, SNAH, Snail 1 Zinc Finger Protein)
Size
£626.00
- SKU:
- 365104
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Met1-Arg264 of human SNAI1, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- SNAI1 is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2. A single transcript of 1.9 kb was detected in several human fetal tissues, with highest expression in kidney. The SNAI1 open reading frame encodes a deduced 264aa protein containing 4 zinc finger motifs that show 87.1% identity to mouse Snai1.SNAI1 expression is higher in normal breast epithelial cell and stromal fibroblast cell lines than in breast cancer cell lines. They showed that SNAI1 interacts with a regulatory region near promoter I.3 of the human aromatase gene, resulting in repression of promoter I.3 and in downregulation of aromatase expression in normal breast tissue. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice