365086
Slit2 (Slit Homolog 2, SLIL3)
Size
£581.00
- SKU:
- 365086
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Cys1408-Cys1519 of mouse Slit2 expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- Slit2 plays a vital role in axon guidance by signaling through Robo receptors. Recent evidence suggests that Slit2 protein may function in other settings because human and Xenopus Slit2 has been shown to inhibit leukocyte chemotaxis. SLIT2 protein is a putative ligand for the ROBO receptors. ROBO1 is inactivated by promoter region hypermethylation in <20% of human cancers. Slit2 is an excellent candidate tumor suppressor gene for colorectal cancer, and that it epigenetically silenced in >40% of lung and breast tumors. SLIT2 promoter region methylation was found in 23 (72%) of 32 primary colorectal cancers. In contrast, normal colorectal mucosa from the same patients exhibited significantly lower levels of SLIT2 promoter region hypermethylation. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:50-400 Immunocytochemistry: 1:50-500 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 ELISA: 1:100-1:5000 Optimal dilutions to be determined by the researcher. AA Sequence: MGHHHHHHSG SEF- CQM IKCKHGKCRL SGVGQPYCEC NSGFTGDSCD REISCRGERI RDYYQKQQGY AACQTTKKVS RLECRGGCAG GQCCGPLRSK RRKYSFECTD GSSFVDEVEK VVKCGCARC Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice