365080
SLC30A3 (Solute Carrier Family 30, Member 3, ZNT3, Zinc Transporter 3)
Size
£626.00
- SKU:
- 365080
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Arg286-Ala388 of human SLC30A3, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- By screening a human temporal cortex cDNA library with a mouse zinc transporter-3 (Znt3) cDNA, Palmiter et al. (1996) cloned cDNAs encoding ZNT3. The predicted 388aa ZNT3 protein has 6 transmembrane domains, with the N and C termini located in the cytoplasm. The human and mouse ZNT3 proteins are 86% identical. Northern blot and RT-PCR analyses of mouse tissues detected Znt3 transcripts only in brain and testis. By in situ hybridization of mouse brain, Znt3 mRNA was most abundant in the hippocampus and cerebral cortex. Immunohistochemical analysis of brain using antibodies against Znt3 detected protein expression in a pattern similar to that of histochemically localized vesicular zinc. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:50-400 Immunocytochemistry: 1:50-500 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 ELISA: 1:100-1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice