365047
SHH (Hedgehog Homolog, Sonic, HHG1, HLP3, HPE3, SMMCI, SHHN, SHH-N)
Size
£537.00
- SKU:
- 365047
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Ser237-Arg399 of rat SHH, expressed in E. coli.
- Format:
- Supplied as a liquid in 0.01M PBS, pH7.4, 0.05% Proclin-300, 50% glycerol.
- Extra Details:
- Sonic hedgehog (Shh) is a member of a small family of secreted proteins that are essential for development in both vertebrates and invertebrates. In mammals, there are three related hedgehog genes (sonic; desert; Indian) which share approximately 60 % aa identity. Human Shh is synthesized as a 437 aa precursor that contains a 24 aa signal sequence and a 413 aa mature region. The mature region is autocatalytically processed into a nonglycosylated, 20kD, 174 aa N-terminal fragment (Shh-N), and a catalytic-processing, glycosylated, 34kD, 239 aa C-terminal fragment. The 20kD Shh-N fragment is the core of the active hedgehog molecule. It begins with a Cys and ends with a Gly, and both amino acids are sites of lipid modification. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice