365023
SEMA7A (Semaphorin 7A, CD108, CDw108, H-SEMA-K1, H-Sema-L, JMH, SEMAK1, SEMAL, Semaphorin 7A, GPI Membrane Anchor, John Milton Hagen Blood Group, Semaphorin-K1, Semaphorin-L)
Size
£495.00
- SKU:
- 365023
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Arg318-Met566 of mouse SEMA7A, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- SEMA7A is a membrane-bound semaphorin that associates with cell surfaces via a glycosylphosphatidylinositol (GPI) linkage. SEMA7A is also known as the John-Milton-Hagen (JMH) blood group antigen, an 80kD glycoprotein expressed on activated lymphocytes and erythrocytes. Czopik et al. (2006) found that T cells from immunized Sema7a -/- mice had increased proliferative responses to antigen that were not attributable to Sema7a deficiency on macrophages or dendritic cells. Sema7a -/- mice were prone to die at the onset of experimental autoimmune encephalomyelitis (EAE) and had higher clinical EAE scores compared with wildtype littermates. Delayed-type hypersensitivity responses were also enhanced in Sema7a -/- mice. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:50-400 Immunocytochemistry: 1:50-500 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 ELISA: 1:100-1:5000 Optimal dilutions to be determined by the researcher. AA Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- RVY GVFSNPWNYS AVCVYSLGDI DRVFRTSSLK GYHMGLPNPR PGMCLPKKQP IPTETFQVAD SHPEVAQRVE PMGPLKTPLF HSKYHYQKVV VHRMQASNGE TFHVLYLTTD RGTIHKVVES GDQDHSFVFN IMEIQPFHRA AAIQAISLDA DRRKLYVTSQ WEVSQVPLDM CEVYSGGCHG CLMSRDPYCG WDQDRCVSIY SSQRSVLQSI NPAEPHRECP NPKPDEAPLQ KVSLARNSRY YLTCPM Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice