365020
SEMA4D (Semaphorin 4D, CD100, A8, BB18, GR3, M-Sema G, M-Sema-G, SEMAJ, Coll-4, Sema Domain, Immunoglobulin Domain(Ig), Transmembrane Domain(TM)and Short Cytoplasmic Domain 4D)
Size
£537.00
- SKU:
- 365020
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Leu145-Gly494 of rat SEMA4D, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- CD100 was first identified as a cell surface protein of resting T cells; previous studies had shown that it was involved in lymphocyte activation. Hall et al. (1996) cloned CD100 from a cDNA library of phytohemagglutinin-activated human T cells. Sequence analysis showed that CD100 encodes an 863aa polypeptide containing a secretory signal sequence, a single transmembrane domain, an immunoglobulin (Ig)-like domain, and a conserved 500aa sema domain. Thus, Hall et al. (1996) stated that CD100 is a member of the semaphorin family and the first semaphorin believed to be involved in the immune system. See SEMA3Fand SEMA3B. Northern blot analysis revealed that CD100 is expressed primarily as a 4.5-kb message in a variety of tissues, both hematopoietic and nonhematopoietic. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:50-400 Immunocytochemistry: 1:50-500 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 ELISA: 1:100-1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice