SDHB (Succinate Dehydrogenase Complex Subunit B, SDH1, SDH, Ip, Iron Sulfur, Iron-sulfur subunit of complex II, Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial)

Size

£537.00

SKU:: 365012
Purity:: Purified by Protein A and peptide affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Glu97-Ala282 of mouse SDHB, expressed in E. coli.
Format:: Supplied as a liquid in 0.01M PBS, pH7.4, 0.05% Proclin-300, 50% glycerol.
Extra Details:: Complex II in mitochondria, of which succinate dehydrogenase (EC 1.3.99.1) is a component, has 4 subunits. In order of decreasing molecular mass, they are the flavoprotein (SDHA), the iron sulfur protein (SDHB), and the 2 integral membrane proteins, SDHC and SDHD. Kita et al. (1990) cloned and sequenced the iron sulfur protein subunit. A clone was isolated from a human liver cDNA library. The open reading frame encodes a 25aa protein. The amino acid sequence showed approximately 94% homology with that of bovine heart. Au et al. (1995) described the complete genomic clone for the gene encoding the iron sulfur protein subunit. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.