RNASE11 (Ribonuclease A11, C14orf6)

Size

£544.00

SKU:: 364920
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Ala18-Leu199 of human RNASE11, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: RNASE11 belongs to the pancreatic ribonuclease family. Ribonuclease A (RNase A) is a pancreatic ribonuclease that cleaves single-stranded RNA. Bovine pancreatic RNase A is one of the classic model systems of protein science. RNase A is a relatively small protein. It can be characterized as a two-layer alpha +Beta protein that is folded in half to resemble a taco, with a deep cleft for binding the RNA substrate. The first layer is composed of three alpha helices from the N-terminal half of the protein. The second layer consist of three Beta-hairpins arranged in two Beta-sheets. The hairpins 61-74 and 105-124 form a four-stranded, antiparallel Beta-sheet that lies on helix 3. The longest Beta-hairpin 79-104 mates with a short Beta-strand to form a three-stranded, antiparallel Beta-sheet that lies on helix 2. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunocytochemistry: 1:50-500 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice