RIPK1 (Receptor Interacting Serine Threonine Kinase 1, RIP, Receptor-Interacting Protein, Cell death protein RIP, Serine/threonine-protein kinase RIP)

Size

£577.00

SKU:: 364910
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Met1-Ala179 of rat 1, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: Two cell surface cytokine receptors, FAS and tumor necrosis factor (TNF) receptor, trigger apoptosis by natural ligands or specific agonist antibodies. Both receptors contain a conserved intracellular death domain. Using a yeast 2-hybrid screen with the cytoplasmic domain of FAS as bait, Stanger et al. (1995) isolated partial cDNAs encoding a receptor-interacting protein which they designated RIP. They used the partial cDNAs to isolate mouse cDNAs corresponding to the entire Rip coding region. Hsu et al. (1996) isolated a full-length human RIP cDNA. They reported that the predicted 671aa RIP protein contains an N-terminal protein kinase domain, a C-terminal death domain, and a unique internal region that they called the intermediate domain. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice