RAGE (Renal Tumor Antigen, MOK, RAGE1, MAPK/MAK/MRK Overlapping Kinase)

Size

£577.00

SKU:: 364854
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Met1-Tyr237 of rat RAGE, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: Tumor antigens recognized by cytolytic T cells (CTLs) can be classified into 4 groups: shared tumor-specific antigens encoded by MAGE-type genes that are silent in most normal tissues except testis and expressed in many tumors; differentiation antigens that are also expressed in normal cells; antigens resulting from tumor-specific point mutations; and antigens overexpressed in tumor cells. By screening cells expressing both HLA-B7 and kidney tumor cell RNA with autologous CTLs, followed by anchored PCR analysis, Gaugler et al. (1996) isolated a cDNA encoding RAGE. RT-PCR analysis detected expression only in retina and in a number of tumor cell types. One putative RAGE open reading frame encodes a 40aa protein and includes the peptide recognized by HLA-B7-restricted CTLs. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice