364846
RAB37 (RAB37, Member RAS Oncogene Family, Ras-related protein Rab-37)
Size
£517.00
- SKU:
- 364846
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Ser19-Cys220 of human RAB37, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- Using yeast 2-hybrid analysis with mouse Rab5 as bait, Masuda et al. (2000) identified Rab37 from a mouse mast cell line. The deduced Rab37 protein contains sequence motifs conserved in other low molecular mass GTPases. It shares 74% and 47% aa sequence identity with rat RAB26 and mouse Rab8 (RAB8A). Northern blot analysis showed expression of 2.3- and 1.2-kb Rab37 transcripts exclusively in the MC9 mast cell line. Immunoprecipitation followed by Western blot analysis of a panel of mouse tissues showed that a 34kD band corresponding to the Rab37 protein is expressed exclusively in bone marrow mast cells. Expression of GFP-tagged Rab37 in bone marrow mast cells showed that Rab37 localizes to secretory granules. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice