PZP (Pregnancy Zone Protein, CPAMD6, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 6)

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£537.00

SKU:: 364841
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Leu1246-Ala1495 of mouse PZP, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: Pregnancy zone protein (PZP), one of the major pregnancy-associated plasma proteins, was described by Smithies (1959) who used zone-electrophoresis in starch gels. PZP is a prominent constituent of late-pregnancy sera. In healthy, nonpregnant females and in males, PZP is present in trace amounts only: females, 10-30 mg/l; males, less than 10 mg/l. During pregnancy, PZP levels may reach 1000-1400 mg/l just before term. Sottrup-Jensen et al. (1984) showed that PZP closely resembles alpha-2-macroglobulin in structure. Both have a quaternary structure of 2 covalently bound 180kD subunits which are further noncovalently assembled into a tetramer of 720kD. aa sequence of the 2 proteins are extensively homologous. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice