364784
PSMD9 (Proteasome 26S Subunit, Non ATPase 9, Rpn4, p27, 26S proteasome regulatory subunit p27, 26S proteasome non-ATPase regulatory subunit 9)
Size
£544.00
- SKU:
- 364784
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Ser2-Arg223 of human PSMD9, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- PSMD9 encodes a non-ATPase subunit of the 19S regulator.Sequence analysis predicted that the 222aa Bridge-1 protein shares 98% aa similarity with human PSMD9 over the first 184aa but diverges in the C terminus Bridge-1 contains a PDZ-like domain from aa138-178, forming 3 beta sheets and 2 alpha helices. SDS-PAGE analysis showed that Bridge-1 is expressed as a 28kD protein, close to the deduced value of 25kD. Using Bridge-1 cDNA as probe, Northern blot analysis detected a 1.0-kb transcript in all rat and human tissues tested, with highest expression in pancreas, testis, kidney, and liver. Immunocytochemistry assessment demonstrated predominant nuclear localization of Bridge-1, with lower levels in cytoplasm. Applications: Suitable for use in Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice