PRKX (Protein Kinase, X-Linked, PKX1, cAMP-dependent protein kinase catalytic subunit PRKX)

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£577.00

SKU:: 364751
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Trp49-Phe358 of rat PRKX, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: PKX1 appeared to encode a novel type of protein kinase that is related to the catalytic subunit of cAMP-dependent protein kinases and has striking homology to the DC2 protein kinase of Drosophila melanogaster. PKX1 is part of a family of at least 4 genes or pseudogenes, of which 3 map to the human sex chromosomes. In contrast to all other genes from the X-specific region of Xp22.3, PKX1 has a homolog on Yp (PRKY) rather than Yq. This suggests that the single pericentric inversion event hypothesized to have occurred during primate evolution is not sufficient to explain the present X/Y homology pattern of Xp22.3. This suggests that the PKX1 gene, besides harboring a previously described hotspot for illegitimate Xp/Yp recombination, contains additional sequences which predispose the X chromosome to breakage events. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice