364736
PPT1 (Palmitoyl Protein Thioesterase 1, PPT, CLN1, INCL, Ceroid-Lipofuscinosis,Neuronal 1,Infantile, Palmitoyl-protein hydrolase 1)
Size
£537.00
- SKU:
- 364736
- Application:
- WB
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Asp28-Lys279 of mouse PPT1, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- PPT1 is a small glycoprotein involved in the catabolism of lipid-modified proteins during lysosomal degradation. The encoded enzyme removes thioester-linked fatty acyl groups such as palmitate from cysteine residues. Defects in this gene are a cause of infantile neuronal ceroid lipofuscinosis 1 (CLN1, or INCL) and neuronal ceroid lipofuscinosis 4 (CLN4). Two transcript variants encoding different isoforms have been found for this gene. The cDNA predicted a 306aa polypeptide that contains a 25aa signal peptide, 3 N-linked glycosylation sites, and consensus motifs characteristic of thioesterases. Northern blot analysis revealed ubiquitous expression of a single 2.5-kb mRNA, with highest expression in lung, brain, and heart. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. AA Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- DPP SPPPLVIWHG MGDSCCNPMS MGVIKKMVEK EIPGIYVLSL EIGKNMMEDV ENSFFLNVNV QVNMVCQILE KDPKLQQGYN AIGFSQGGQF LRAVAQRCPT PPMMTLISVG GQHQGVFGLP RCPGESSHIC DFIRKSLNAG AYSKLVQERL VQAQYWHDPI KESVYRNYSI FLADINQERC VNESYKKNLM ALKKFVMVKF FNDSIVDPVD SEWFGFYRSG QAKETIPLQE STLYTEDRLG LKKMDKAGK Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice