364718
POSTN (Periostin, OSF-2, PDLPOSTN, PN, Osteoblast Specific Factor 2)
Size
£577.00
- SKU:
- 364718
- Application:
- WB
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Arg659-Gln810 of rat POSTN, fused to N-terminal His-Tag expressed in E. coli.
- Format:
- Supplied as a liquid in 0.01M PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
- Extra Details:
- By screening human placenta and osteosarcoma cDNA libraries with mouse Postn as probe, they cloned 2 variants of human POSTN. One variant encodes a deduced 779aa protein with an apparent molecular mass of 87.0kD, and the other encodes a deduced 836aa protein with an apparent molecular mass of 93.3kD. POSTN contains a typical signal sequence, followed by a cysteine-rich domain, a 4-fold repeat structure of about 150aa, and a C-terminal domain. Mouse and human POSTN share 89.2% amino acid identity overall and 90.1% identity in their mature forms. Northern blot analysis of a mouse osteoblastic cell line detected a 3.4-kb Postn transcript. RNA dot blot analysis detected expression of Postn in primary mouse osteoblasts and in lung, but not in any other tissues examined. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice