364707
POLa1 (Polymerase DNA Directed Alpha 1, POLA, p180, DNA polymerase alpha catalytic subunit p180)
Size
£537.00
- SKU:
- 364707
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Ala1209-Ser1465 of mouse POLa1, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- Hanaoka et al. (1985) complemented a temperature-sensitive defect in murine POLA by fusing the defective mouse cells with human cells. A gene present on Xpter-q22 was responsible for correcting the defect. Wang et al. (1985) used a monoclonal antibody that distinguishes human from rodent DNA polymerase alpha to study human-rodent cell hybrids. They mapped the gene to a site near the junction of Xp21.3 and Xp22.1 (perhaps Xp21.12-p21.11) and showed that it is not expressed in an inactive X chromosome. They concluded that POLA is distal to DMD and CGD but proximal to STS. They predicted that mosaic nullisomy in a female resulting from deletion of this gene could not exist and that hemizygosity for deletion of the gene in a male would be lethal because of the essentiality of the gene to the catalytic activity of DNA polymerase alpha. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice