364696
PLVAP (Plasmalemma Vesicle Associated Protein, FELS, PV-1, PV1, gp68, Fenestrated-Endothelial Linked Structure Protein, Plasmalemma vesicle protein 1)
Size
£626.00
- SKU:
- 364696
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Arg123-Lys393 of human PLVAP, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- PLVAP is a type II integral membrane protein with a short N-terminal cytoplasmic tail, a single-span transmembrane domain, and a large C-terminal extracellular domain consisting mostly of alpha helices. It also contains 2 coiled-coil domains predicted to contribute to dimer formation. The extracellular domain has 4 N-glycosylation sites, a proline-rich region, and regular spacing of cysteine residues. The deduced 439aa mouse Plvap protein contains a consensus casein kinase II phosphorylation site that is not found in the human protein. Rat Plvap contains 2 casein kinase II phosphorylation sites. Northern blot analysis revealed a single PLVAP transcript of about 2.4 kb in pancreas, kidney, placenta, muscle, lung, heart, and liver. No signal was detected in brain or testis. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. AA Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- RVIYTNNQ RYMAAIILSE KQCRDQFKDM NKSCDALLFM LNQKVKTLEV EIAKEKTICT KDKESVLLNK RVAEEQLVEC VKTRELQHQE RQLAKEQLQK VQALCLPLDK DKFEMDLRNL WRDSIIPRSL DNLGYNLYHP LGSELASIRR ACDHMPSLMS SKVEELARSL RADIERVARE NSDLQRQKLE AQQGLRASQE AKQKVEKEAQ AREAKLQAEC SRQTQLALEE KAVLRKERDN LAKELEEKKR EAEQLRMELA IRNSALDTCI KTK Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice