364684
PLEKHA1 (Pleckstrin Homology Domain Containing Family A, Member 1, TAPP1, Tandem PH Domain Containing Protein-1, PH domain-containing family A member 1)
Size
£537.00
- SKU:
- 364684
- Application:
- IHC-Fr
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Thr105-Leu351 of mouse PLEKHA1, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- The last 3 C-terminal aa of TAPP1 are predicted to interact with PDZ domain-containing proteins. TAPP1 and TAPP2 share 58% identity over the first 300aa, which encompass both PH domains. There is little homology in the C termini. Northern blot analysis detected a 4.0-kb transcript expressed in all tissues examined, with highest levels in skeletal muscle, spleen, lung, thymus, and placenta. TAPP1 possessed a 5-fold higher affinity for PtdIns(3,4)P2 than TAPP2. Mutation of the conserved arg212 to leucine within the PPBM of the second PH domain of TAPP1 reduced or eliminated PtdIns(3,4)P2 binding. The N-terminal PH domain contributed to the affinity with which the C-terminal PH domain bound to PtdIns(3,4)P2. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice