364671
PLA2G10 (Phospholipase A2, Group X, GXPLA2, GX sPLA2, sPLA2-X, Group 10 secretory phospholipase A2, Phosphatidylcholine 2-acylhydrolase 10)
Size
£626.00
- SKU:
- 364671
- Application:
- IHC-Fr
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Glu32-Asp165 of human PLA2G10, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- The A2 phospholipases (PLA2s; EC 3.1.1.4) catalyze the release of fatty acids from phospholipids and play a role in a wide range of physiologic functions. Cupillard et al. (1997) cloned a novel secretory PLA2, termed GXSPLA2, from a human fetal lung cDNA library. The sequence encodes a 165aa polypeptide, including the 42aa signal peptide. Northern blot analysis revealed that GXSPLA2 expresses an 0.8-kb mRNA in pancreas, lung, and colon and a 1.5-kb mRNA in spleen, thymus, and peripheral blood leukocytes. Expression studies showed that the cDNA encodes an active phospholipase which was secreted into the culture medium. Cupillard et al. (1997) used in situ hybridization to map the PLA2G10 gene to human chromosome 16p13.1-p12. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice