PKlg (Protein Kinase Inhibitor Gamma, PKI-G, CAMP-Dependent Protein Kinase Inhibitor Gamma)

Size

£581.00

SKU:: 364663
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Met1-Ser76, of mouse PKlg, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: Protein kinase inhibitor Gamma is a member of the PKA inhibitor family. Studies of a similar protein in mice suggest that this protein acts as a potent competitive PKA inhibitor, and is a predominant form of PKA inhibitors in various tissues. Three alternatively spliced transcript variants encoding the same protein have been reported.Most of the amino acid residues in the domain of PKI-gamma that showed affinity for the catalytic subunit are conserved, and PKI-gamma also has a region highly similar to the consensus PKI nuclear export signal. Northern blot analysis detected a 1.3-kb transcript in all tissues examined, with highest expression in heart, skeletal muscle, and testis. The authors verified the presence of PKI-gamma protein in mouse tissues by purifying it from heart using DEAE chromatography. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice