PKD1 (Protein Kinase D1, PKC-MU, PKCM, PK-D1, PRKCM, Protein Kinase C,Mu)

Size

£581.00

SKU:: 364657
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Glu349-Gly612 of mouse PKD1, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: By screening a placenta cDNA library using a PCR fragment derived from the second conserved domain within the catalytic site of ser/thr protein kinases, Johannes et al. obtained a full-length cDNA encoding PKC-mu. The deduced 912aa PRKCM protein has a calculated molecular mass of 102kD. PRKCM has 2 characteristic cysteine-rich repeats, which are conserved among nearly all members of the PKC family, a conserved ATP-binding consensus sequence in the kinase domain, and the invariate aspartate essential for kinase activity. Northern blot analysis detected a single PRKCM transcript of 3.8 kb at low, constitutive levels in all tissues examined. The authors verified PRKCM kinase activity by transfection of a PRKCM expression vector and detecting changes in protein phosphorylation patterns by Western blot analysis. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice