PK2 (Prokineticin 2, PK-2, Bv8, MIT1, KAL4, Protein Bv8 Homolog)

Size

£482.00

SKU:: 364651
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Ile30-Gln128 of human PK2, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Extra Details:: Prokineticin 2 is a novel 81aa peptide with a complex folding structure including five disulphide linkages. It is a mammalian homolog to the mamba snake venom protein mamba intestinal toxin 1 and the frog protein Bv8, and has been demonstrated to be a paracrine mediator of gastrointestinal smooth muscle contractility.PK2 is expressed throughout the mammalian organism; however, in the brain, its expression is specifically concentrated in the suprachiasmatic nucleus (SCN). PK2 is rhythmically expressed in the SCN, transcriptionally regulated by clock control genes, and has the capacity to strongly suppress nocturnal locomotor activity. PK2 is one of the first neuropeptides that appears to directly convey circadian rhythm signaling to other CNS structures and, therefore, it may be a critical output molecule regulating biological rhythms. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:100-1:5000 Western Blot: 1:50-400 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 Immunocytochemistry: 1:50-500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice