364646
PIP (Prolactin Induced Protein, GCDFP15, GPIP4, BRST2, SABP, gp17, Gross cystic disease fluid protein 15, Prolactin-induced protein, Secretory actin-binding protein)
Size
£533.00
- SKU:
- 364646
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Asn41-Thr139 of rat PIP, fused to N-terminal His-Tag expressed in E. coli.
- Format:
- Supplied as a liquid in 0.01M PBS, pH7.4, 0.05% Proclin-300, 50% glycerol.
- Extra Details:
- The hormonally responsive prolactin-inducible protein gene is expressed in benign and malignant breast tumor tissues and in some normal exocrine organs such as sweat, salivary, and lacrimal glands. The PIP gene is 7 kb long with 4 exons ranging from 106 bp to 223 bp. PIP is overexpressed in 80% of primary and metastatic breast cancers, is duplicated in the breast carcinoma cell line T47D. The 2 copies are organized as a large palindrome, lying 'in loco' on one chromosome 7. The FRA7I fragile site lies 2 Mb telomeric to the PIP gene and sets the distal end of the repeated sequence. Breakage at the FRA7I site was repaired by 1 cycle of the breakage--fusion-bridge (BFB) cycle mechanism, resulting in amplification of the PIP gene. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice