PDHX (Pyruvate Dehydrogenase Complex Component X, E3BP, proX, PDX1, OPDX, DLDBP, E3-binding protein, Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex)

Size

£537.00

SKU:: 364589
Purity:: Purified by Protein A and peptide affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to Leu222-Met471 of mouse PDHX, expressed in E. coli.
Format:: Supplied as a liquid in 0.01M PBS, pH7.4, 0.05% Proclin-300, 50% glycerol.
Extra Details:: The PDHX gene encodes component X of the pyruvate dehydrogenase (PDH) complex. For a detailed description of the pyruvate dehydrogenase complex. The mammalian PDH complex differs from that in E. coli and from the other mammalian alpha-keto acid dehydrogenases by the presence of a 53kD protein called protein X. Component X binds to the E3 component of the PDH complex. The open reading frame encodes an 501aa protein sequence that is 20% identical with that encoded by the yeast PDX1 gene and 40% identical with that encoded by the lipoacyltransferase component E2 of the PDH (E2), and includes a lipoyl-bearing domain. Sequence comparisons showed that the PDHX protein contains an E3-binding domain. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. AA Sequence: MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LKQMGKITE SRPASAPPPS LSASVPPQAT AGPSYPRPMT PPVSIPGQPN AAGTFTEIPA SNIRRVIAKR LTESKSTVPH AYATADCDLG AVLKVRRDLV KDDIKVSVND FIIRAAAVTL KQMPGVNVTW DGEGPKQLPS VDISVAVATD KGLITPIIKD AAAKGIQEIA DSVKVLSKKA RDGKLMPEEY QGGSFSISNL GMFGIDEFAA VINPPQACIL AVGRFRPVLK LTEDEEGNPQ LQQHQLITVT M Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice