364576
PDE10A (Phosphodiesterase 10A, cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A)
Size
£626.00
- SKU:
- 364576
- Application:
- WB
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Asn508-Asp779 of human PDE10A, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- Various cellular responses are regulated by the second messengers cAMP and cGMP. Phosphodiesterases, such as PDE10A, eliminate cAMP- and cGMP-mediated intracellular signaling by hydrolyzing the cyclic nucleotide to the corresponding nucleoside 5-prime monophosphate. The deduced 779aa protein has a calculated molecular mass of 88.4kD and contains cGMP-binding sequences and a catalytic domain. Northern blot analysis detected a 10-kb PDE10A transcript in several tissues, with highest levels in fetal brain and kidney, in putamen and caudate nucleus of adult brain, and in thyroid and testis. A 4-kb transcript was also detected in thyroid and testis. mRNA dot blot analysis showed a similar PDE10A expression pattern. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice