P4Ha3 (Prolyl-4-Hydroxylase Alpha Polypeptide III , Procollagen-Proline, 2-Oxoglutarate 4-Dioxygenase(Proline 4-Hydroxylase), Alpha Polypeptide III)

Size

£531.00

SKU:: 364503
Purity:: Purified by Protein A and peptide affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Recombinant protein corresponding to aa227-544 of human P4Ha3, expressed in E. coli.
Format:: Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
Extra Details:: P4HA3 encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. P4HA3 has all residues necessary to bind the peptide substrate, to coordinate Fe(2+) and the 2-oxoglutarate cosubstrate, and for enzymatic activity. Processed P4HA3 contains 525aa and shares 91% and 94%aa identity with rat and mouse P4ha3, respectively, and 35% and 37% identity with P4HA1 and P4HA2, respectively. Northern blot analysis detected a 2.7-kb P4HA3 transcript. Highest expression was in placenta, adult liver, and fetal skin, with lower levels in fetal liver, lung, and muscle. RT-PCR detected P4HA3 expression in all tissues examined, with highest expression in placenta and fetal kidney, liver, and lung. Expression of P4HA3 was much lower than that of P4HA1. Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry (paraffin): 5-20ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice