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364449

NUP155 (Nucleoporin 155kD, N155)

Size

£544.00

SKU:
364449
Purity:
Purified by Protein A and peptide affinity chromatography.
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Rabbit
Immunogen:
Recombinant protein corresponding to aa1154-1379 of human NUP155, expressed in E. coli.
Format:
Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
Extra Details:
Nuclear pore complex protein Nup155shares 91% sequence identity with the rat homolog, including an identical stretch of 6aa (GVRLYF). Like the rat homolog, it is a member of the nonrepetitive/WGA-negative class of nucleoporins. Neither possesses the typical repeat sequences of FG, FXFG, or GLFG found in most types of vertebrate nucleoporins. Unlike the rat protein, the human protein contains a leucine zipper in its N terminus. Like other nucleoporins, it contains numerous potential phosphorylation sites. Northern blot analysis detected a major 5.4-kb transcript in all tissues tested, but predominantly in heart, placenta, liver, and skeletal muscle, and a minor 4.7-kb transcript in some tissues tested, consistent with the finding of 2 alternative polyadenylation sites in the cDNA. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 0.01-5ug/ml Immunohistochemistry (Paraffin): 5-20ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Shipping Conditions:
Blue Ice