364430
NT-ProANP (N-Terminal Pro-Atrial Natriuretic Peptide, NT-Pro-ANP, N-ANP)
Size
£482.00
- SKU:
- 364430
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to aa25-123 of human NT-ProANP, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- ANP is a hormone important in maintaining body fluid and sodium homeostasis. In cardiomyocytes, human ANP is synthesized as a 151aa prepropeptide. After the signal peptide is removed by the signal peptidase, pro-ANP is stored in the dense granules of the cell. On stimulation, pro-ANP is released from the dense granules and activated on the cell surface by proteolytic cleavage at residue arginine 98, generating an NH2-terminal propeptide and a 26aa COOH-terminal peptide that is biologically active. Plasma concentration of N-terminal atrial natriuretic peptide (Nt-proANP) is strongly directly related to salt sensitivity.In contrast to Nt-proANP, Nt-proBNP is not related to salt sensitivity. Salt sensitivity, as estimated by Nt-proANP, seems to be reduced in type 2 diabetes. Both N-ANP and N-BNP identifies a greater number of patients at risk of death or heart failure than either peptide alone. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.01-2ug/ml Immunohistochemistry: 5-20ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice