364417
NR1D2 (Nuclear Receptor Subfamily 1, Group D, Member 2, HZF2, BD73, EAR-1r, RVR, Rev-ErbA Beta, Orphan nuclear hormone receptor BD73, Rev-erb alpha-related receptor, V-erbA-related protein 1-related)
Size
£577.00
- SKU:
- 364417
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to aa249-578 of rat NR1D2, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- Rev-ErbA beta is a member of the Rev-ErbA family of transcription factors. Rev-erbBeta, like Rev-erbalpha, belongs to the nuclear receptor superfamily and can modulate gene expression by directly binding to their promoters.Rev-erbBeta is similar to Rev-erbalpha in its protein structure and function as a transcriptional repressor. The crystal structure of an unliganded Rev-erbBeta ligand-binding domain (LBD) has been resolved (see figure to the right) and shows an extremely small ligand-binding pocket. However, Rev-erbBeta has been shown to interact with heme, which appears important for its function. The structure of Rev-erbBeta complexed with heme (see figure to the left) shows a substantial movement of helices 1 (N-terminus ) and 11 (C-terminus ) which opens up a large binding pocket in the interior of the protein that is able to accommodate this ligand. Applications: Suitable for use in Western Blot, Immunohistochemistry, Immunocytochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 0.01-2ug/ml Immunohistochemistry: 5-20ug/ml Immunocytochemistry: 5-20ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice