364380
NNE (Enolase, Non Neuronal, ENO1, ENO1L1, MPB1, PPH, Alpha Enolase, Enolase 1, Phosphopyruvate hydratase, Plasminogen-binding protein, 2-phospho-D-glycerate hydro-lyase, C-myc promoter-binding)
Size
£524.00
- SKU:
- 364380
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to aa78-241 of human NNE, expressed in E. coli.
- Format:
- Supplied as a liquid in 0.01M PBS, pH7.4, 0.05% Proclin-300, 50% glycerol.
- Extra Details:
- Enolase 1, more commonly known as alpha-enolase, is a glycolytic enzyme expressed in most tissues, one of the isozymes of enolase. It is a homodimer composed of 2 alpha subunits. Its gene, the ENO1, also encodes the Myc-binding protein-1(MBP1), which downregulates the activity of c-myc protooncogene. Alpha-enolase is the longer form (48kD) localized in both cytoplasm and nuclei, while MBP1 is shorter (37kD) and is found mostly in the nuclei. Alpha-enolase has been identified as an autoantigen in Hashimotos encephalopathy. Single studies have also identified it as an autoantigen associated with severe asthma and a putative target antigen of anti-endothelial cell antibody in Behets disease. Reduced expression of the enzyme has been found in the corneas of people suffering from keratoconus. Applications: Suitable for use in Immunocytochemistry/Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.1-2ug/ml Immunohistochemistry (paraffin): 5-20ug/ml Immunocytochemistry/Immunofluorescence: 5-20ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice