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364374

NMES1 (Normal Mucosa Of Esophagus Specific 1, C15orf48, Protein FOAP-11)

Size

£544.00

SKU:
364374
Purity:
Purified by Protein A and peptide affinity chromatography.
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Rabbit
Immunogen:
Recombinant protein corresponding to aa1-83 from human NMES1 expressed in E. coli.
Format:
Supplied as a liquid in PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
Extra Details:
NMES1 shares 31 to 34%aa identity with the mouse, bovine, and human NADH-ubiquinone oxidoreductase MLRQ subunit. Northern blot analysis detected a transcript of 0.9 kb in esophagus, stomach, small intestine, colon, and placenta. Very weak expression was detected in lung, and no expression was detected in several other tissues. Immunohistochemical staining revealed expression localized to nuclei of the basal cell layer of normal esophagus. In situ hybridization of embryonic mice revealed expression in the ossification center and spinal cord 8 days postcoitum. Expression extended throughout bone tissue and vertebrae from this stage until birth. Other sites of abundant expression included the developing brain, intestine, and stomach. Weak expression was detected in the developing mouse lung. Applications: Suitable for use in ELISA, Immunohistochemistry, Immunofluorescence and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Paraffin): 5-20ug/ml Immunofluorescence (IC): 5-20ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Shipping Conditions:
Blue Ice