364368
NKRF (NFKB Repressing Factor, ITBA4, NRF, NF-Kappa B Repressing Factor, Transcription factor NRF)
Size
£626.00
- SKU:
- 364368
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Met1-Val296 of human NKRF, expressed in E. coli.
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
- Extra Details:
- NKRF encodes a transcription factor that interacts with specific negative regulatory elements (NREs) to mediate transcriptional repression of certain NK-kappa-B-responsive genes. The protein localizes predominantly to the nucleolus with a small fraction found in the nucleoplasm and cytoplasm. The deduced 388aa protein has a calculated molecular mass of 43.8 kD and contains an N-terminal nuclear localization signal. The C-terminal 92aa of NRF constitute the DNA-binding domain, which has 4 turns and 4 alpha helices, similar to a winged helix-turn-helix DNA-binding motif. Northern blot analysis detected ubiquitous expression of 3.7- and 4.0-kb transcripts that differ in their 3-prime untranslated regions. The longer transcript predominated in most tissues, but the 3.7-kb transcript predominated in brain. Applications: Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:50-400 Immunocytochemistry: 1:50-500 Immunohistochemistry (frozen): 1:50-500 Immunohistochemistry (paraffin): 1:10-100 ELISA: 1:100-1:5000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice