364357
NIN (Ninein, GSK3B Interacting Protein, Glycogen synthase kinase 3 beta-interacting protein, GSK3B-interacting protein)
Size
£626.00
- SKU:
- 364357
- Application:
- WB
- Purity:
- Purified by Protein A and peptide affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Recombinant protein corresponding to Met1-Glu153 of human NIN, expressed in E. coli.
- Format:
- Supplied as a liquid in 0.01M PBS, pH 7.4, 0.05% Proclin-300, 50% glycerol.
- Extra Details:
- Ninein is one of the proteins important for centrosomal function. This protein is important for positioning and anchoring the microtubules minus-ends in epithelial cells. Localization of this protein to the centrosome requires three leucine zippers in the central coiled-coil domain. Multiple alternatively spliced transcript variants that encode different isoforms have been reported.The deduced 2,047aa protein has a calculated molecular mass of 239kD. NIN contains an N-terminal GTP-binding site, a large coiled-coil domain with 4 leucine zipper motifs, and a C-terminal domain responsible for GSK binding. Unlike mouse Nin, it does not have an N-terminal Ca(2+)-binding site. Northern blot analysis detected a 9.0-kb transcript in all tissues examined and a 7.0-kb transcript predominantly in heart and skeletal muscle. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice